resource source identifier goat anti-spp1 (osteopontin) r&d systems cat#af808  (R&D Systems)

Journal: bioRxiv

Article Title: Fluid-Niche and Microglial Signatures Prime Robust Intraventricular Macrophage Response to Blood During Brain Development

doi: 10.1101/2025.10.02.679906

Figure Lengend Snippet: ( A ) Myeloid cell population clustering from E16.5 ChP. ( B ) CellChat visualization of inferred outgoing signals from epithelial, mesenchymal, and endothelial cells to ChP macrophages. ( C ) Incoming vs. outgoing signal strength across populations. ( D ) Heatmap of selected differentially expressed genes from bulk RNA sequencing of sorted P7 stromal vs epiplexus macrophages. ( E ) Representative image of an E14.5 brain sections stained with SPP1. Scale bar = 10 µm. ( F ) UMAP of other myeloid population signatures reflected in the various ChP macrophage populations. ( G ) Schematic of choroid plaque development from E12.5-E16.5. ( H ) Representative image of an E12.5 brain section. Scale bar = 200 µm. Inset: Magnified view of choroid plaque. Scale bar = 50 µm. ( I ) Representative image of an E17.5 and E18.5 brain section showing the residual choroid plaque and underlying macrophages. Scale bar = 250 µm; 25 µm for inset. ( J ) Representative image of a P0 brain section showing the roughly equivalent region following the crossing of the corpus callosum as well as the lateral ventricle and ChP. Scale bar = 250 µm; 25 µm for inset.

Article Snippet: 488 (ThermoFisher, 53-0443-82, 1:100), Goat anti-OPN (SPP1) (Novus Biologicals, AF808, 1:100), Goat anti-GPNMB (R&D Systems, AF2330, 1:500), Rat anti-LY76 (Ter119)(Abcam, ab91113, 1:500), Chicken anti-IBA1 (Synaptic Systems, 234-009, 1:500), Rabbit anti-AQP1 (Millipore Sigma, AB2219, 1:100), Rabbit anti-MRC1 (CD206) (Abcam, ab64693, 1:250), Rabbit anti-HMOX1 (Proteintech, 10701-1-AP, 1:250).

Techniques: RNA Sequencing, Staining

Journal: eLife

Article Title: Multisensory gaze stabilization in response to subchronic alteration of vestibular type I hair cells

doi: 10.7554/elife.88819

Figure Lengend Snippet: Figure 3. Immuno-labelling of HC in the central regions of the horizontal SCC ampulla and striolar region of the utricule Macula. (A, C) Immunolabelling of type I HC (Spp1 + and CASPR1+), type II HC (Calre +), or all HC (Myo7a) for the SHAM, IDPN W6 and IDPN W12 groups in the central ampulla of the horizontal canal (A1) and central utricular maculae (C1). Cell count at W6 and W12 in the central horizontal ampulla (A2 and A3) and central utricular maculae (C2 and C3) for individual mice (Kruskal Wallis test). (B1, D1) Individual number of central Spp1 + type I HC, CASPR1 + type I HC and Calre +

Article Snippet: The primary antibodies used were rabbit anti- Myosin VIIa (Myo7a) (Proteus Biosciences, #25–6790), mouse anti- contactin- associated protein (CASPR1) (Neuromab #75–001), guinea pig anti calretinin (Synaptic Systems #214–104) and goat anti- osteopontin (Spp1) (R&D Systems #AF808).

Techniques: Cell Counting